GARNet Service : Progress and Results of Applications 

 

 

 

 

 Rothamsted Research is one if eight institutes sponsored by the biotechnology and biological sciences research council

 

 

 

 

 

 

 

 

 

 

Application Number

Applicant

Analysis to be completed

Number of Plant Lines

Status

Report

MB00/001 (Report)

Ottoline Leyser

Carotenoids

 

 

ABA

3 (max4-1, max4-2 and col wild type)

Completed

 

 

?

MB00/002

Nick Smirnoff

Carbohydrates

Amino Acids

Organic Acids

Phenylpropanoids

NMR

5 (vtc 1-1,vtc 2-1, vtc 3-1, vtc 4-1 and col wild type)

In progress?

In progress?

In progress?

Completed

Completed

MB00/003 (Report)

John Mundy

Carotenoids

2 (rbz and ler)

Completed

MB00/004 (Report)

Mike Holdsworth

Fatty Acids

3 (Comatose mutants and wt)

Completed

MB00/005 (Report)

Keith Lindsey

Hormones

Cytokinin (hydra/polaris)

Lipids (hydra)

Fatty Acids

Sterols (hydra)

Glycerides (hydra)

PS/PI/PE/SM

Glycolipids

Auxin IAA hydra/polaris

5 ( Hydra 1-2, Hydra 2, Polaris, C24 and ws wild type)

Polaris, C24, wt and Hydra 1-2 and 2 plants grown and material stored March 2003.

 

 

 

 

Completed

 

 

 

 

MB00/006 (Report)

Gary Loake

JA/SA

8 (Activation tagged lines)

Completed

MB00/007 (Report)

Simon Turner

Phenylpropanoids

Flavonoids

NMR

Carbohydrates

Amino Acids

Organic Acids

Sterols

PS/PI/PE/SM

3 (irx 3, irx 4 and Ler wild type)

Completed

Completed

Completed

Work being carried out on

irx5-3, irx2-1 and irx 1-1. See application MB03/004.

 

MB01/002 (Report)

Dianna Bowles

Phenylpropanoids

28 (UGT + Ri lines + col wt)

Completed

MB01/003 (Report)

Ian Furner

SAH

2 (Hog – 1 + Ler wt)

Completed

MB01/006 (Report)

Dianna Bowles

Phenylpropanoids

4 (UGT +  col wt)

Completed

MB01/007

Mike Bevan

NMR

GC/MS

7 (hsr and col wild type, 2 growth stages + 2 treatments)

Completed

Completed

MB01/009 (Report)

Liz Rylott

Phenylpropanoids

NMR

GC/MS

7 (16, 64, 122, 92, 195 and  2 controls wta and wtb)

Completed

Completed

In Progress?

 

MB01/010

Steve Smith

NMR

Carbohydrates

Amino Acids

Organic Acids

8 (ms. Ws, icl, iclrev, d1, d2, d3, and wild type)

Completed

More Material to be supplied so further analysis can be carried out. Analysis Completed.

MB02/001

Matthew Terry/

Alison Smith

NMR

GC/MS

HPLC

2 (hy1 and wild type)

Completed

MB02/002

Murray Grant

NMR

GC/MS

1 (Col – 5, 3 time points and 5 treatments)

Completed

 

MB02/003 (Report)

Simon Turner

Polyamines

3 (cov–1, cov-2 and Ler wt)

Completed

MB03/001 (Report)

Christine Foyer

Phenylpropanoids

Flavonoids

2 (Col-0 and vtc 1-1)

Completed

MB03/002

Repeat of MB00/002

Nick Smirnoff

NMR

5 (Col-0, vtc 1-1, 2-1, 3-1 and 4-1 to two growth stages – rosette and flowering).

Completed

(Report in Progress)

 

MB03/003

Nick Smirnoff

NMR

4 (Col-0, vtc 2-1, 2-2 and 2-3)

Seed collected awaiting sieving and then growth

 

MB03/004

Simon Turner

NMR

6 (Ler-0 wt, irx 1-1, irx 2-1, irx 3, irx 4 and irx 5-3 analysis of stems and leaves separately)

Completed

(Report in Progress)

 

MB03/005

Johnathan Napier

(Michalea)

NMR

4 (Col-0, P450 12/5.2, u/4.1 and 5/5.4)

Completed

(Report in Progress)

 

MB04/001

Johnathan Napier

(Michaela)

NMR

3 Treatments Col-0 (untreated, cis jasmone and methyl jasmonate)

Plant Growth completed. Ground waiting NMR analysis.

 

MB04/002

Johnathan Napier

(Louise Michaelson)

NMR

8 (Col-0, S3.10, S8.8, S6.1, A19.2, A28.3, A27.1 and A21.1)

Plant growth completed. Material to be freeze dried week beginning 22.3.04

 

MB04/003

Alison Smith

NMR

2 (col-0 and lin2, two treatments light and dark. 4 harvesting times for col-0 and 8 harvesting times for lin2)

Material received freeze dried 11.3.04. Waiting grinding and NMR analysis.

 

MB04/004

Alison Smith

NMR

6 (col, Landsberg, hy1,hy2, lin2 and Gun5)

Seed received 11.3.04. Waiting to be grown.

 

MB04/005

Johnathan Napier

(Michaela)

HPLC

Phenylpropanoids

4 (col-0, P450 5/1.3, P450 11/4.5 and P450 12/5.3

Plant material being harvested

 

MB04/006

(ref: MeT04003DB)

Dianna Bowles

Global NMR Fingerprinting and subsequent targeted analysis

10

Seed received ting to Plant growth in progress